大包装询价 产品介绍 评论(0)
宿主来源
Mouse抗原名称
Siglec-2/CD22分子别名
B-cell receptor CD22, B-lymphocyte cell adhesion molecule (BL-CAM), Sialic acid-binding Ig-like lectin 2 (Siglec-2), T-cell surface antigen Leu-14免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P20273克隆号
S-565-1抗体类型
Mouse mAb抗体同种型
IgG1,k应用
FCM, ICC反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| ICC | 1:250 |
| FCM | 1:200-1:2000 |
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. In mice, it has been shown that CD22 blockade restores homeostatic microglial phagocytosis in aging brains. Because CD22 is restricted to B cells, it is an excellent target for immunotherapy of B cell malignancies. There are several mechanisms by which this can be achieved, namely monoclonal antibodies, bispecific antibodies, antibody-drug conjugates, radioimmunoconjugates or CAR-T cells.
流式分析
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, left) / Daudi (Human Burkitt's lymphoma lymphoblast, right) cells labelling Siglec-2 antibody at 1/200 dilution (1 μg) / (red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: JurkatFlow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling Siglec-2/CD22 antibody at 1/2000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ separately. Gated on total viable cells.
免疫细胞化学
ICC shows positive staining in Daudi cells. Anti- Siglec-2/CD22 Mouse antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in Jurkat cells. Anti-Siglec-2/CD22 Mouse antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
评论(0)