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Non-muscle Myosin IIB/MYH10 Recombinant Rabbit mAb (S-299-3)

Myosin-10,Cellular myosin heavy chain,type B,Myosin heavy chain 10,Myosin heavy chain,non-muscle IIb,NMMHC-B,NMMHC II-b,NMMHC-IIB,NM-IIB

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0302

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Non-muscle Myosin IIB/MYH10
分子别名

Myosin-10, Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b; NMMHC-IIB, NM-IIB
免疫原

Synthetic Peptide
Accession

P35580
克隆号

S-299-3
抗体类型

Recombinant mAb
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Rb
纯化方式

Protein A
浓度

0.1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:4000 Hu, Ms, Rt
IHC 1:100

应用	稀释度	推荐种属
WB	1:4000	Hu, Ms, Rt
IHC	1:100

背景介绍

Myosin-10 also known as myosin heavy chain 10 or non-muscle myosin IIB (NM-IIB) is a protein that in humans is encoded by the MYH10 gene. Non-muscle myosins are expressed in a wide variety of tissues, but NM-IIB is the only non-muscle myosin II isoform expressed in cardiac muscle, where it localizes to adherens junctions within intercalated discs. NM-IIB is essential for normal development of cardiac muscle and for integrity of intercalated discs. Mutations in MYH10 have been identified in patients with left atrial enlargement.

免疫印迹
- WB result of non-muscle Myosin IIB/MYH10 Rabbit mAb
  Primary antibody: non-muscle Myosin IIB/MYH10 Rabbit mAb at 1/4000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 229 kDa
  Observed MW: 230 kDa
- WB result of non-muscle Myosin IIB/MYH10 Rabbit mAb
  Primary antibody: non-muscle Myosin IIB/MYH10 Rabbit mAb at 1/4000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 229 kDa
  Observed MW: 230 kDa
- WB result of non-muscle Myosin IIB/MYH10 Rabbit mAb
  Primary antibody: non-muscle Myosin IIB/MYH10 Rabbit mAb at 1/4000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 229 kDa
  Observed MW: 230 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebellum. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-Non-muscle Myosin IIB/MYH10 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.