Neutrophil Elastase Recombinant Rabbit mAb (S-R230)

Bone marrow serine protease,Elastase-2,Human leukocyte elastase (HLE),Medullasin,PMN elastase,ELANE,ELA2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0301

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Neutrophil Elastase
分子别名

Bone marrow serine protease, Elastase-2, Human leukocyte elastase (HLE), Medullasin, PMN elastase, ELANE, ELA2
Accession

P08246
克隆号

S-R230
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC 1:500-1:2000
IP 1:50

应用	稀释度
WB	1:1000
IHC	1:500-1:2000
IP	1:50

背景介绍

Neutrophil elastase is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Neutrophil elastase is secreted by neutrophils during inflammation, and destroys bacteria and host tissue. It also localizes to neutrophil extracellular traps (NETs), via its high affinity for DNA, an unusual property for serine proteases. Neutrophil elastase is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Mutations of the ELANE gene cause cyclic and severe congenital neutropenia, which is a failure of neutrophils to mature. In 2019 study was confirmed that ELANE deletion does not cause neutropenia.

免疫印迹
- WB result of Neutrophil Elastase Rabbit mAb
  Primary antibody: Neutrophil Elastase Rabbit mAb at 1/1000 dilution
  Lane 1: Jurkat whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Lane 3: U937 whole cell lysate 20 µg
  Lane 4: HL-60 whole cell lysate 20 µg
  Negative control: Jurkat whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
  (This blot was developed with high sensitivity substrate)
免疫沉淀
- Neutrophil Elastase Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Neutrophil Elastase in 0.4 mg U937 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Neutrophil Elastase Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: U937 whole cell lysate 20 µg (Input)
  Lane 2: Neutrophil Elastase Rabbit mAb IP in U937 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in U937 whole cell lysate
  Predicted MW: 29 kDa
  Observed MW: 29 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human bone marrow. Anti-Neutrophil Elastase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Neutrophil Elastase antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.