Neurofilament M Recombinant Rabbit mAb (S-501-31)

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货号： S0B0300

Datasheet COA

价格： ￥600
规格：
10μl25μl100μl1ml
数量：

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产品规格

宿主来源

Rabbit
抗原名称

Neurofilament M
分子别名

Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cytoskeleton
Accession

P07197
克隆号

S-501-31
抗体类型

Recombinant mAb
应用

IHC-P, WB, IP, IF
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC	1:500
IP	1:50
IF	1:2000

背景介绍

Neurofilament medium polypeptide (NF-M) is a protein that in humans is encoded by the NEFM gene. Neurofilaments are type IV intermediate filament heteropolymers composed of light (NEFL), medium (this protein), and heavy (NEFH) chains. Neurofilaments comprise the exoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage.

免疫印迹
- WB result of Neurofilament M Rabbit mAb
  Primary antibody: Neurofilament M Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Lane 2: mouse cerebellum lysate 20 µg
  Negative control: mouse liver lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 102 kDa
  Observed MW: 160 kDa
- WB result of Neurofilament M Rabbit mAb
  Primary antibody: Neurofilament M Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Negative control: rat liver lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 102 kDa
  Observed MW: 160 kDa
免疫沉淀
- Neurofilament M Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Neurofilament M in 0.4 mg mouse cerebellum lysate.
  Western blot was performed on the immunoprecipitate using Neurofilament M Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: mouse cerebellum lysate 20 µg (Input)
  Lane 2: Neurofilament M Rabbit mAb IP in mouse cerebellum lysate
  Lane 3: Rabbit monoclonal IgG IP in mouse cerebellum lysate
  Predicted MW: 102 kDa
  Observed MW: 160 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- Neurofilament M antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cerebellum. Anti- Neurofilament M antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti- Neurofilament M antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human cerebellum. Anti-Neurofilament M antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.