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Histone H4 Recombinant Rabbit mAb (S-499-30)

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0297
规格
数量
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H4

  • 细胞定位

    Nucleus

  • Accession

    P62805

  • 克隆号

    S-499-30

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    ChIP ,
    ICFCM ,
    IHC-P ? ,
    ICC ? ,
    WB ,
    IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Fu, Dr, Pl, Ys

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
IHC 1:2000
ICC 1:500
ICFCM 1:50
ChIP 1:20-1:50
背景介绍

  • Histone H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosome of the 'beads on a string' organization. Histone H4 is an important protein in the structure and function of chromatin, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

  • 免疫印迹

    • WB result of Histone H4 Rabbit mAb
      Primary antibody: Histone H4 Rabbit mAb at 1/1000 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa
      Exposure time: 180 s

    • WB result of Histone H4 Rabbit mAb
      Primary antibody: Histone H4 Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa
      Exposure time: 180 s

    • WB result of Histone H4 Rabbit mAb
      Primary antibody: Histone H4 Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 11 kDa
      Observed MW: 14 kDa
      Exposure time: 180 s

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Histone H4 antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫沉淀

    • Histone H4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Histone H4 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using Histone H4 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: Histone H4 Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 11 kDa
      Observed MW: 14 kDa
      （This blot was developed with high sensitivity substrate）

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Histone H4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H4 Recombinant Rabbit mAb (S-499-30) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30, GAPDH, MYOD1,
      AFM, SAT-α and SAT-2 in Histone H4 Recombinant Rabbit
      mAb (S-499-30)-immunoprecipitated sample.

  • 斯达特公司的抗体，可以回收利用几次？

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