Histone H4 Recombinant Rabbit mAb (S-499-30)
Histone H4,H4,H4C1,H4/A,H4FA,HIST1H4A,H4C2,H4/I,H4FI,HIST1H4B,H4C3,H4/G,H4FG,HIST1H4C,H4C4,H4/B,H4FB,HIST1H4D,H4C5,H4/J,H4FJ,HIST1H4E,H4C6,H4/C,H4FC,HIST1H4F,H4C8,H4/H,H4FH,HIST1H4H,H4C9,H4/M,H4FM,HIST1H4I,H4C11,H4/E,H4FE,HIST1H4J,H4C12,H4/D,H4FD,HIST1H4K,H4C13,H4/K,H4FK,HIST1H4L,H4C14,H4/N,H4F2,H4FN,HIST2H4,HIST2H4A,H4C15,H4/O,H4FO,HIST2H4B,H4C16,H4-16,HIST4H4
货号： S0B0297
- 价格： ￥400
- 规格：
- 数量：
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宿主来源
Rabbit抗原名称
Histone H4分子别名
H4C6免疫原
Synthetic Peptide细胞定位
NucleusAccession
P62805克隆号
S-499-30抗体类型
Recombinant mAb应用
ICFCM, IHC-P, ICC, WB, IP反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Fu, Dr, Pl, Ys纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC | 1:2000 |
| ICC | 1:500 |
| ICFCM | 1:50 |
| IP | 1:50 |
Histone H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosome of the 'beads on a string' organization. Histone H4 is an important protein in the structure and function of chromatin, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
免疫印迹
WB result of Histone H4 Rabbit mAb
Primary antibody: Histone H4 Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDaWB result of Histone H4 Rabbit mAb
Primary antibody: Histone H4 Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDaWB result of Histone H4 Rabbit mAb
Primary antibody: Histone H4 Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Histone H4 antibody at 1/50 dilution (1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
Histone H4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Histone H4 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Histone H4 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Histone H4 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 11 kDa
Observed MW: 14 kDa
（This blot was developed with high sensitivity substrate）
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-Histone H4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
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