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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H3 (mono methyl K36) Recombinant Rabbit mAb (S-R211)

H3K36me

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0296
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (mono methyl K36)

  • 分子别名

    H3K36me

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-R211

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    甲基化

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICFCM

    ChIP

    IHC-P ?

    ICC ?

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Hu
    IHC 1:500
    ICC 1:500 Hu
    ChIP 1:20~1:50 Hu
背景介绍

  • H3K36me is an epigenetic modification to the DNA packaging protein Histone H3, specifically, the mono-methylation at the 36th lysine residue of the histone H3 protein. The methylation of H3K36 has particularly had effects in transcriptional repression, alternative splicing, dosage compensation, DNA replication and repair, DNA methylation, and the transmission of the memory of gene expression from parents to offspring during development.

  • 免疫印迹

    • WB result of Histone H3 (mono methyl K36) Rabbit mAb
      Primary antibody: Histone H3 (mono methyl K36) Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa
      Exposure time: 180 s

    • WB result of Histone H3 (mono methyl K36) Rabbit mAb
      Primary antibody: Histone H3 (mono methyl K36) Rabbit mAb at 1/1000 dilution
      Lane 1: mouse liver lysate 20 µg
      Lane 2: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa
      Exposure time: 180 s

    • WB result of Histone H3 (mono methyl K36) Rabbit mAb
      Primary antibody: Histone H3 (mono methyl K36) Rabbit mAb at 1/1000 dilution
      Lane 1: rat liver lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 17 kDa
      Observed MW: 17 kDa
      Exposure time: 180 s

  • 流式分析

    • Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Histone H3 (mono methyl K36) antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor ® 488 was used as the secondary antibody.

  • 免疫沉淀

    • Histone H3 (mono methyl K36) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Histone H3 (mono methyl K36) in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using Histone H3 (mono methyl K36) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: Histone H3 (mono methyl K36) Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 17 kDa
      Observed MW: 17 kDa
      Exposure time: 80 s

  • 斑点杂交

    • Dot blot result of Histone H3 (mono methyl K36) Rabbit mAb
      Lane 1: H3K36me peptide
      Lane 2: H3K36me2 peptide
      Lane 3: H3K36me3 peptide
      Lane 4: H3K36un peptide
      Primary antibody: Histone H3 (mono methyl K36) Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Exposure time: 17 s

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-Histone H3 (mono methyl K36) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Histone H3 (mono methyl K36) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (mono methyl K36) Recombinant Rabbit mAb (S-R211) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of RPL30, GAPDH, MYOD1, AFM, SAT-α and SAT-2 in Histone H3 (mono methyl K36) Recombinant Rabbit mAb (S-R211) - immunoprecipitated sample.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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