Pan Trk Recombinant Rabbit mAb ,PBS Only (SDT-400-39)

Trk A + Trk B + Trk C

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货号 S0B0262P

规格

100μg1mg

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Pan Trk
分子别名

Trk A + Trk B + Trk C
免疫原

Synthetic Peptide
细胞定位

Membrane
Accession

P04629, Q16620, Q16288
克隆号

SDT-400-39
抗体类型

Rabbit mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

1 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS
储存条件

12 months from date of receipt / reconstitution, 4 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度

WB 1:1000
IHC-P 1:250

应用	稀释度
WB	1:1000
IHC-P	1:250

背景介绍

Neurotrophic tyrosine kinase receptor (NTRK) is a family of 3 proto-oncogenes including NTRK1, NTRK2, and NTRK3 which encode Trk A, Trk B, and Trk C proteins, respectively [PMID: 28719467]. Pan-TRK antibodies have been used to detect gene fusions in diverse types of tumors [PMID: 37017998].

免疫印迹
- WB result of Pan Trk Rabbit mAb
  Primary antibody: Pan Trk Rabbit mAb at 1/1000 dilution
  Lane 1: mouse kidney lysate 20 µg
  Lane 2: mouse spleen lysate 20 µg
  Lane 3: mouse brain lysate 20 µg
  Negative control: mouse kidney lysate; mouse spleen lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 120 kDa
- WB result of Pan Trk Rabbit mAb
  Primary antibody: Pan Trk Rabbit mAb at 1/1000 dilution
  Lane 1: rat kidney lysate 20 µg
  Lane 2: rat spleen lysate 20 µg
  Lane 3: rat brain lysate 20 µg
  Negative control: rat kidney lysate; rat spleen lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 87 kDa
  Observed MW: 120 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human diffuse astrocytoma. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.