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Rabbit抗原名称
Pan Trk分子别名
Trk A + Trk B + Trk C免疫原
Synthetic Peptide细胞定位
MembraneAccession
P04629, Q16620, Q16288克隆号
SDT-400-39抗体类型
Rabbit mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Av纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:250 |
Neurotrophic tyrosine kinase receptor (NTRK) is a family of 3 proto-oncogenes including NTRK1, NTRK2, and NTRK3 which encode Trk A, Trk B, and Trk C proteins, respectively [PMID: 28719467]. Pan-TRK antibodies have been used to detect gene fusions in diverse types of tumors [PMID: 37017998].
免疫印迹
WB result of Pan Trk Rabbit mAb
Primary antibody: Pan Trk Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse kidney lysate; mouse spleen lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 120 kDaWB result of Pan Trk Rabbit mAb
Primary antibody: Pan Trk Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Lane 3: rat brain lysate 20 µg
Negative control: rat kidney lysate; rat spleen lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 120 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse astrocytoma. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat liver. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat spleen. Anti-Pan Trk antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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