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S-RMab® GAPDH Recombinant Rabbit mAb (S-240-147)

Glyceraldehyde-3-phosphate dehydrogenase

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0261
规格
数量
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产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GAPDH

  • 分子别名

    Glyceraldehyde-3-phosphate dehydrogenase

  • 细胞定位

    Cytoplasm, Cytoskeleton, Nucleus

  • Accession

    P04406, P16858

  • 克隆号

    S-240-147

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P ? ,
    ICC ? ,
    WB

  • 反应种属 ?

    Hu, Ms, Rt, Zf, Mk, Ck

  • 预测反应种属
    (反应种属缩写表)

    Hamster (95%), Mink (95%), Pig (95%), Squirrel (95%),Dog (95%), Cat (95%), Sheep (95%), Quail (95%), Bovine (95%), Chicken (95%)

  • 纯化方式

    Protein A

  • 浓度

    1 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:10000-1:200000 Hu, Ms, Rt, Mk, Zf, Ck
IHC-P 1:4000 Hu, Ms, Rt
ICC 1:1000 Hu
背景介绍

  • Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous and highly conserved multifunctional protein that plays a central role in cellular metabolism, primarily catalyzing the sixth step of glycolysis where it converts glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate while simultaneously reducing NAD+ to NADH. Beyond its canonical function in energy production, GAPDH exhibits remarkable "moonlighting" activities depending on its subcellular localization, participating in diverse processes such as DNA repair, transcriptional regulation, vesicular transport, and the initiation of apoptosis under stress conditions like oxidative damage or nitrosative stress. Due to its constitutive and relatively stable expression across various tissues and experimental conditions, GAPDH has historically been widely used as a loading control in techniques like Western blotting and RT-PCR, although recent studies caution against this practice without validation since its levels can fluctuate significantly in response to specific metabolic states or pathological conditions. Structurally, it typically functions as a homotetramer, and its involvement in several neurodegenerative diseases, including Huntington's and Alzheimer's, highlights its critical importance in maintaining cellular homeostasis and its potential as a therapeutic target.

  • 免疫印迹

    • WB result of GAPDH Recombinant Rabbit mAb
      Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: MDA-MB-231 whole cell lysate 20 µg
      Lane 4: SK-OV-3 whole cell lysate 20 µg
      Lane 5: PC-3 whole cell lysate 20 µg
      Lane 6: HEK-293 whole cell lysate 20 µg
      Lane 7: A549 whole cell lysate 20 µg
      Lane 8: HepG2 whole cell lysate 20 µg
      Lane 9: PANC-1 whole cell lysate 20 µg
      Lane 10: HT-29 whole cell lysate 20 µg
      Lane 11: Ramos whole cell lysate 20 µg
      Lane 12: Jurkat whole cell lysate 20 µg
      Lane 13: K562 whole cell lysate 20 µg
      Lane 14: A431 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 36 kDa

    • WB result of GAPDH Recombinant Rabbit mAb
      Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: RAW264.7 whole cell lysate 20 µg
      Lane 3: EL4 whole cell lysate 20 µg
      Lane 4: C2C12 whole cell lysate 20 µg
      Lane 5: Neuro-2a whole cell lysate 20 µg
      Lane 6: mouse brain lysate 20 µg
      Lane 7: mouse colon lysate 20 µg
      Lane 8: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 36 kDa

    • WB result of GAPDH Recombinant Rabbit mAb
      Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg
      Lane 3: rat brain lysate 20 µg
      Lane 4: rat colon lysate 20 µg
      Lane 5: rat liver lysate 20 µg
      Lane 6: rat heart lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 36 kDa

    • WB result of GAPDH Recombinant Rabbit mAb
      Primary antibody: GAPDH Recombinant Rabbit mAb at 1/200000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Lane 2: zebrafish lysate 20 µg
      Lane 3: chicken liver lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 36 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human liver. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human spleen. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-GAPDH antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-GAPDH antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of GAPDH in tumor tissues.

    • Expression of GAPDH in human tissues.

    • Expression of GAPDH in mouse & rat tissues.

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