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Human IgG1 F(c) Mouse mAb (S-448-1)

Immunoglobulin heavy constant gamma 1,Ig gamma-1 chain C region,Ig gamma-1 chain C region EU,Ig gamma-1 chain C region KOL,Ig gamma-1 chain C region NIE,IGHG1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0255

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Mouse
抗原名称

Human IgG1 F(c)
分子别名

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
免疫原

Synthetic Peptide
细胞定位

Secreted, Cell membrane
Accession

P01857
克隆号

S-448-1
抗体类型

Mouse mAb
抗体同种型

IgG1
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ELISA

WB
稀释度

应用稀释度

WB 1:1000
IHC 1:5000

应用	稀释度
WB	1:1000
IHC	1:5000

背景介绍

The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].

免疫印迹
- WB result of Human IgG1 F(c) mouse mAb Primary antibody: Human IgG1 F(c) mouse mAb at 1/1000 dilution Lane 1: Human serum lysate 5 µg Secondary antibody: Goat Anti-Mouse IgG F(c), (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 55 kDa
- WB result of Human IgG1 F(c) mouse mAb Primary antibody: Human IgG1 F(c) mouse mAb at 1/1000 dilution Lane 1: IgG recombinant protein lysate 20 ng Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 55 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.