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宿主来源
Mouse抗原名称
Human IgG1 F(c)分子别名
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1免疫原
Synthetic Peptide细胞定位
Cell membrane, SecretedAccession
P01857克隆号
S-448-1抗体类型
Mouse mAb抗体同种型
IgG1应用
IHC-P, ELISA, WB反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC | 1:5000 |
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
免疫印迹
WB result of Human IgG1 F(c) mouse mAb Primary antibody: Human IgG1 F(c) mouse mAb at 1/1000 dilution Lane 1: Human serum lysate 5 µg Secondary antibody: Goat Anti-Mouse IgG F(c), (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 55 kDa
WB result of Human IgG1 F(c) mouse mAb Primary antibody: Human IgG1 F(c) mouse mAb at 1/1000 dilution Lane 1: IgG recombinant protein lysate 20 ng Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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