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GBP1 Recombinant Rat mAb (S-R186)

Guanylate-binding protein 1,GTP-binding protein 1,GBP-1,HuGBP-1,hGBP1,Guanine nucleotide-binding protein 1,Interferon-induced guanylate-binding protein 1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0254

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rat
抗原名称

GBP1
分子别名

Guanylate-binding protein 1; GTP-binding protein 1; GBP-1; HuGBP-1; hGBP1; Guanine nucleotide-binding protein 1; Interferon-induced guanylate-binding protein 1
细胞定位

Golgi apparatus membrane, Cytoplasm, Secreted, Cell membrane
Accession

P32455
克隆号

S-R186
抗体类型

Rat mAb
抗体同种型

IgG1
反应种属 ?

Hu
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IP 1:50
IHC 1:100
ICC 1:500

应用	稀释度
WB	1:1000
IP	1:50
IHC	1:100
ICC	1:500

背景介绍

A single cluster of seven human GBP genes (GBP1-GBP7) is found on chromosome 1q22.2. Human GBP1 is secreted from cells without the need of a leader peptide, and has been shown to exhibit antiviral activity against Vesicular stomatitis virus and Encephalomyocarditis virus, as well as being able to regulate the inhibition of proliferation and invasion of endothelial cells in response to IFN-gamma [PMID: 16936281].

免疫印迹
- WB result of GBP1 Rat mAb
  Primary antibody: GBP1 Rat mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with IFN-γ (50 ng/ml 16 hr) whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
  Exposure time: 20 s
免疫沉淀
- GBP1 Rat mAb at 1/200 dilution (1 µg) immunoprecipitating GBP1 in 0.4 mg Jurkat whole cell lysate.
  Western blot was performed on the immunoprecipitate using GBP1 Rat mAb at 1/1000 dilution.
  Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution.
  Lane 1: Jurkat whole cell lysate 20 µg (Input)
  Lane 2: GBP1 Rat mAb IP in Jurkat whole cell lysate
  Lane 3: Rat monoclonal IgG1 IP in Jurkat whole cell lysate
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-GBP1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-GBP1 antibody was used at 1/100 dilution, followed by a Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC analysis of HeLa cells treated with IFNγ (50ng/ml, 16h) (top panel) and untreated HeLa cells (below panel). Anti- GBP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).