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MLKL Recombinant Rat mAb (SDT-R195)

Mixed lineage kinase domain-like protein,hMLKL

货号： S0B0253

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rat

  • 抗原名称

    MLKL

  • 分子别名

    Mixed lineage kinase domain-like protein, hMLKL

  • 免疫原

    N/A

  • 细胞定位

    Membrane, Cytoplasm, Cell membrane, Nucleus

  • Accession

    Q8NB16

  • 克隆号

    SDT-R195

  • 抗体类型

    Rat mAb

  • 抗体同种型

    IgG1

  • 应用

    ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
WB 1:1000
ICC 1:50
背景介绍

  • Mixed lineage kinase domain-like (MLKL) is the executioner in the caspase-independent form of programmed cell death called necroptosis. Receptor-interacting serine/threonine protein kinase 3 (RIPK3) phosphorylates MLKL, triggering MLKL oligomerization, membrane translocation and membrane disruption [PMID: 34698396].

  • 免疫印迹

    • WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: HUVEC whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Lane 4: HeLa whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa

    • WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: RAW 264.7 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa

    • WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: C6 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa

  • 免疫细胞化学

    • ICC shows positive staining in HT-29 cells. Anti-MLKL antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.

    • Negative control: ICC shows negative staining in Raji cells. Anti-MLKL antibody was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI

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