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Rat抗原名称
MLKL分子别名
Mixed lineage kinase domain-like protein, hMLKL细胞定位
Cytoplasm, Nucleus, Membrane, Cell membraneAccession
Q8NB16克隆号
SDT-R195抗体类型
Rat mAb抗体同种型
IgG1反应种属 ?
Hu, Ms, Rt纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:50 Hu
Mixed lineage kinase domain-like (MLKL) is the executioner in the caspase-independent form of programmed cell death called necroptosis. Receptor-interacting serine/threonine protein kinase 3 (RIPK3) phosphorylates MLKL, triggering MLKL oligomerization, membrane translocation and membrane disruption [PMID: 34698396].
免疫印迹
WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: Raji whole cell lysate 20 µg Lane 2: HUVEC whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Lane 4: HeLa whole cell lysate 20 µg Negative control: Raji whole cell lysate Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa
WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: RAW 264.7 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa
WB result of MLKL Rat mAb Primary antibody: MLKL Rat mAb at 1/1000 dilution Lane 1: C6 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 54 kDa Observed MW: 54 kDa
免疫细胞化学
ICC shows positive staining in HT-29 cells. Anti-MLKL antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
Negative control: ICC shows negative staining in Raji cells. Anti-MLKL antibody was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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