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宿主来源
Rat抗原名称
PES1分子别名
Pescadillo homolog细胞定位
Nucleus, NucleolusAccession
O00541克隆号
SDT-R197抗体类型
Rat mAb抗体同种型
IgG1反应种属 ?
Hu浓度
0.4 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 WB 1:4000 IHC 1:400 ICC 1:100 ICFCM 1:500 IP 1:40
Pescadillo homolog is a protein that in humans is encoded by the PES1 gene [PMID: 8985183]. It is a nuclear protein that is involved in embryonic development, ribosome synthesis, DNA replication, and cell cycle progression [PMID: 31814761].
免疫印迹
WB result of Pescadillo Rat mAb Primary antibody: Pescadillo Rat mAb at 1/4000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Lane 3: HEK-293 whole cell lysate 20 µg Lane 4: HCT 116 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rat IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 68 kDa Observed MW: 70 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PES1 at 1/500 dilution (0.1 μg) / (red) compared with a Rat monoclonal IgG isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
PES1 Rat mAb at 1/40 dilution (1 µg) immunoprecipitating PES1 in 0.4 mg HEK-293 whole cell lysate.
Western blot was performed on the immunoprecipitate using PES1 Rat mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate 20 µg (Input)
Lane 2: PES1 Rat mAb IP in HEK-293 whole cell lysate
Lane 3: Rat monoclonal IgG1 IP in HEK-293 whole cell lysate
Predicted MW: 68 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-PES1 antibody was used at 1/400 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-PES1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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