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PEG10 Recombinant Rabbit mAb (SDT-442-60)

Retrotransposon-derived protein PEG10,Embryonal carcinoma differentiation-regulated protein,Mammalian retrotransposon-derived protein 2,MEF3-like protein 1,Paternally expressed gene 10 protein,Ty3/Gypsy-like protein,MEF3L1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0242

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

PEG10
分子别名

Retrotransposon-derived protein PEG10, Embryonal carcinoma differentiation-regulated protein, Mammalian retrotransposon-derived protein 2, MEF3-like protein 1, Paternally expressed gene 10 protein, Ty3/Gypsy-like protein, MEF3L1
免疫原

Recombinant Protein
细胞定位

Cytoplasm
Accession

Q86TG7
克隆号

SDT-442-60
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
ICC 1:500
IHC-P 1:1000

应用	稀释度
WB	1:1000
ICC	1:500
IHC-P	1:1000

背景介绍

Retrotransposon-derived protein PEG10 is a protein that in humans is encoded by the PEG10 gene. Binds its own mRNA in the 5'-UTR region, in the region near the boundary between the nucleocapsid (NC) and protease (PRO) coding sequences and in the beginning of the 3'-UTR region [PubMed:34413232].

免疫印迹
- WB result of PEG10 Rabbit mAb Primary antibody: PEG10 Rabbit mAb at 1/1000 dilution Lane 1: 293T whole cell lysate 20 µg Lane 2: HeLa whole cell lysate 20 µg Lane 3: HepG2 whole cell lysate 20 µg Negative control: 293T whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 80 kDa Observed MW: 110 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human breast carcinoma. Anti-PEG10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human breast. Anti-PEG10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PEG10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PEG10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-PEG10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human prostate. Anti-PEG10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-PEG10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in 293T cells. Anti-PEG10 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in HepG2 cells. Anti-PEG10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- Negative control: ICC shows negative staining in 293T cells. Anti-PEG10 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4%PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).