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宿主来源
Mouse抗原名称
Human IgG3 F(c)分子别名
Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3免疫原
Synthetic Peptide细胞定位
Secreted, Cell membraneAccession
P01860克隆号
S-460-1抗体类型
Mouse mAb抗体同种型
IgG2b,κ反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.应用
IHC-P ?
ELISA
WB
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:2000
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
免疫印迹
WB result of Human IgG3 F(c) Mouse mAb
Primary antibody: Human IgG3 F(c) Mouse mAb at 1/1000 dilution
Lane 1: Human serum lysate 5 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60kDa
Observed MW: 60kDaWB result of Human IgG3 F(c) Mouse mAb
Primary antibody: Human IgG3 F(c) Mouse mAb at 1/1000 dilution
Lane 1: IgG1 FC recombinant protein lysate 10 ng
Lane 2: IgG2 FC recombinant protein lysate 10 ng
Lane 3: IgG3 FC recombinant protein lysate 10 ng
Lane 4: IgG4 FC recombinant protein lysate 10 ng
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: IgG1 FC、IgG2 FC、IgG4 FC: 27 kDa; IgG3 FC: 33 kDa
Observed MW: IgG3 FC: 40 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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