Human IgG3 F(c) Mouse mAb (S-460-1)

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货号： S0B0229

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产品规格

宿主来源

Mouse
抗原名称

Human IgG3
分子别名

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3
免疫原

Synthetic Peptide
细胞定位

Secreted, Cell membrane
Accession

P01860
克隆号

S-460-1
抗体类型

Mouse mAb
抗体同种型

IgG2b,k
应用

IHC-P, ELISA, WB
反应种属 ?

Hu
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000
IHC-P	1:2000

背景介绍

The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].

免疫印迹
- WB result of Human IgG3 F(c) Mouse mAb
  Primary antibody: Human IgG3 F(c) Mouse mAb at 1/1000 dilution
  Lane 1: Human serum lysate 5 µg
  Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60kDa
  Observed MW: 60kDa
- WB result of Human IgG3 F(c) Mouse mAb
  Primary antibody: Human IgG3 F(c) Mouse mAb at 1/1000 dilution
  Lane 1: IgG1 FC recombinant protein lysate 10 ng
  Lane 2: IgG2 FC recombinant protein lysate 10 ng
  Lane 3: IgG3 FC recombinant protein lysate 10 ng
  Lane 4: IgG4 FC recombinant protein lysate 10 ng
  Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: IgG1 FC、IgG2 FC、IgG4 FC: 27 kDa; IgG3 FC: 33 kDa
  Observed MW: IgG3 FC: 40 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.