IGFBP1 Recombinant Rabbit mAb (SDT-196-47)

Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,Placental protein 12,PP12

价格￥600.00 （现货： 2-5个工作日）

货号 S0B0225

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

IGFBP1
分子别名

Insulin-like growth factor-binding protein 1, IBP-1; IGF-binding protein 1, Placental protein 12, PP12
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P08833
克隆号

SDT-196-47
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度

WB 1:1000
ICC 1:250

应用	稀释度
WB	1:1000
ICC	1:250

背景介绍

Insulin-like growth factor binding proteins (IGFBPs) are a family of proteins binding to Insulin-like growth factors (IGFs), generally including IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, and IGFBP6 [PMID: 30214426]. IGFBP1, a 40 to 50 kDa protein, which plays an indispensable role in normal growth and development, and in the pathophysiology of various tumors. IGFBP-1 has been shown to be associated with the risk of various tumors, and has a vital function in regulating tumor behaviors such as proliferation, migration, invasion and adhesion through different molecular mechanisms. The biological actions of IGFBP-1 in cancer are found to be related to its phosphorylation state, and the IGF-dependent and -independent mechanisms. [PMID: 1648311, PMID: 33841624].

免疫印迹
- WB result of IGFBP1 Rabbit mAb
  Primary antibody: IGFBP1 Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 28 kDa
  Observed MW: 30 kDa
流式分析
- Flow cytometric analysis of HepG2 cells labelling IGFBP1 antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-IGFBP1 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).