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宿主来源
Mouse抗原名称
CD13分子别名
Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M (AP-M), Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, ANPEP, APN, PEPN免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P15144克隆号
S-689-37抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 5 μl per million cells in 100μl volume
Aminopeptidase N (also called CD13) is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. CD13 is also used by some viruses as a receptor to which these viruses bind to and then enter cells. It is a receptor for human coronavirus 229E, feline coronavirus serotype II (FCoV-II), TGEV, PEDV, canine coronavirus genotype II (CCoV-II) as well as several Deltacoronaviruses.
流式分析
Flow cytometric analysis of CD13 expression on THP-1. Cells from the THP-1 (Human monocytic leukemia monocyte, Right) or HEK293 (Human embryonic kidney epithelial cell, Left) was stained with FITC Mouse IgG1, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Human CD13 antibody (Red line histogram) at 1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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