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宿主来源
Mouse抗原名称
CD9分子别名
CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29 (Tspan-29), p24, MIC3, TSPAN29免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P21926克隆号
S-632-33抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.02mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 5 μl per million cells in 100μl volume
CD9 belongs to the cell surface glycoprotein cross -membrane four -protein family, with four cross -membrane domains, a shorter outer domain (ECL1), and a longer extracellular domain (ECL2). It is expressed in a variety of hematopoietic cells and epithelial cells. For example, Pre B cells, B cell subset, activated T cells, basophils, eosinophils, macrophages, megakaryocytes, plasma cells, plasma cell precursors in germinal centers, and platelets. Broadcasting a series of cell processes such as cell adhesion, movement, membrane tissue and signal transfusion. The lowering of CD9 expression is related to the adverse prognosis and progress of various cancers. It is a favorable marker of gallbladder cancer, gastic GIST, malignant cortex and oral squamous cell carcinoma.
流式分析
Flow cytometric analysis of CD9 expression on HCT116. Cells from the HCT116 (Human colorectal carcinoma epithelial cell, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with FITC Mouse IgG1, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Human CD9 antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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