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Rabbit抗原名称
CD163分子别名
M130, Hemoglobin scavenger receptor免疫原
Synthetic Peptide细胞定位
Secreted, Cell membraneAccession
Q86VB7克隆号
S-222-171抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 555性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
IF ?
稀释度
应用 稀释度 IF 1:200
CD163 is a monocyte/macrophage specific marker expressed predominantly on cells which possess strong anti-inflammatory potential. The expression of CD163 is strongly induced by anti-inflammatory mediators such as glucocorticoids and interleukin-10, while being inhibited by pro-inflammatory mediators such as interferon-gamma. CD163-expressing mononuclear phagocytes, as well as soluble CD163, may both take part in downregulating an inflammatory response [PubMed: 22038213].
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD163 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD163 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-CD163 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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