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Ki67 Recombinant Mouse mAb (Alexa Fluor® 594 Conjugate) (S-606-7)

Proliferation marker protein Ki-67,Antigen identified by monoclonal antibody Ki-67,Antigen KI-67,MKI67

价格 ￥1,056.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0205
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    Ki67

  • 分子别名

    Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, MKI67

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P46013

  • 克隆号

    S-606-7

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG1

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 标记

    Alexa Fluor® 594

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

  • 应用

    IF ?

  • 稀释度

    应用 稀释度
    IF 1:100-1:200
背景介绍

  • The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human colon cancer. Anti-Ki67 (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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