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产品规格
宿主来源
Mouse抗原名称
CD73分子别名
5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase, NT5E, NT5, NTE细胞定位
Cell membraneAccession
P21589克隆号
S-715-115抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu纯化方式
Protein A浓度
0.05mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 5 μl per million cells in 100μl volume
背景介绍
NT5E (CD73) is a surface enzyme which is expressed on multiple cells. This enzyme mediates the gradual hydrolysis of the autocrine and paracrine danger signals of ATP and ADP to anti-inflammatory adenosine. Immune suppression mediated by adenosinergic pathways is very important for maintaining immune system homeostasis. Immune suppressive functions of T regulatory cells are also dependent on CD73 expression. CD73 also occurs on anergic CD4 + T cells, thereby maintaining self-tolerance to healthy tissues as well as protecting the fetus from the mother's immune system during pregnancy.
流式分析
- Flow cytometric analysis of Human CD73 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 antibody and either Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD73 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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