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产品规格
宿主来源
Mouse抗原名称
CD21分子别名
Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor (EBV receptor)免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P20023克隆号
S-764-18抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.02mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 5 μl per million cells in 100μl volume
背景介绍
CD21 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d.B cells express CD21 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation. CD21 interacts with CD19 and on mature B cells forms a complex with CD81 (TAPA-1). The CD21-CD19-CD81 complex is often called the B cell co-receptor complex, because CD21 binds to opsonized antigens through attached C3d (or iC3b or C3dg) when the B-cell receptor binds antigen. This results in the B cell having greatly enhanced response to the antigen. Epstein-Barr virus (EBV) can bind CD21, enabling EBV to enter and infect B cells.
流式分析
- Flow cytometric analysis of Human CD21 expression on Raji. Cells from the Raji (Human Burkitt's lymphoma B lymphocyte, Right) or K562 (Human chronic myelogenous leukemia lymphoblast, Left) was stained with Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD21 Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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