所有图片（1/1）
分享产品介绍 评论(0)
产品规格
宿主来源
Mouse抗原名称
CD5分子别名
T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, LEU1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P06127克隆号
S-576-35抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.2mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 5 μl per million cells in 100μl volume
背景介绍
CD5 is a cluster of differentiation expressed on the surface of T cells (various species) and in a subset of murine B cells known as B-1a. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. There is no confirmed ligand for CD5 but there is evidence that CD72, a C-type lectin, may be a ligand or that CD5 may be homophilic, binding CD5 on the surface of other cells.
流式分析
- Flow cytometric analysis of Human CD5 expression on MOLT-4. Cells from the MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Right) or HeLa (Human cervix adenocarcinoma epithelial cell, Left) was stained with Alexa Fluor® 594 Mouse IgG1, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 594 Mouse Anti-Human CD5 Antibody (Red line histogram) at 1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)