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宿主来源
Mouse抗原名称
CD5分子别名
T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, LEU1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P06127克隆号
S-576-35抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
IF ?
稀释度
应用 稀释度 FCM 1:200 IF 1:200
CD5 is a cluster of differentiation expressed on the surface of T cells (various species) and in a subset of murine B cells known as B-1a. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. There is no confirmed ligand for CD5 but there is evidence that CD72, a C-type lectin, may be a ligand or that CD5 may be homophilic, binding CD5 on the surface of other cells.
流式分析
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, left) / Molt-4 (Human lymphoblastic leukemia T lymphoblast, Right) cells labelling CD5 (Alexa Fluor® 488 Conjugate) antibody at 1/200 dilution (1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Negative control: HeLa
免疫荧光
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD5 (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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