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宿主来源
Rabbit抗原名称
CD8α分子别名
Lyt-2免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P01731克隆号
S-142-52抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
IF ?
稀释度
应用 稀释度 IF 1:200 FCM 1:250
CD8α encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
流式分析
Flow cytometric analysis of mouse primary splenocytes labeling CD8α (Alexa Fluor® 594 Conjugate) at 1/250 dilution (right panel) compared with a Rabbit IgG, monoclonal Isotype Control (left panel).
Then cells were stained with CD4 - APC/Cy7 separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
免疫荧光
IF shows positive staining in paraffin-embedded mouse thymus. Anti-CD8α (Alexa Fluor® 594 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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