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Rabbit抗原名称
E-Cadherin分子别名
Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, CD324, Uvomorulin, L-CAM免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P12830克隆号
S-438-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
HRP性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.应用
WB
稀释度
应用 稀释度 WB 1:1000
E-cadherin is a transmembrane glycoprotein which connects epithelial cells together at adherens junctions. In normal cells, E-cadherin exerts its tumour suppressing role mainly by sequestering β-catenin from its binding to LEF (Lymphoid enhancer factor)/TCF (T cell factor) which serves the function of transcribing genes of the proliferative Wnt signaling pathway. Despite the ongoing debate on whether the loss of E-cadherin is the cause or effect of epithelial-mesenchymal transition (EMT), E-cadherin functional loss has frequently been associated with poor prognosis and survival in patients of various cancers. The dysregulation of E-cadherin expression that leads to carcinogenesis happens mostly at the epigenetic level but there are cases of genetic alterations as well. E-cadherin expression has been linked to the cellular functions of invasiveness reduction, growth inhibition, apoptosis, cell cycle arrest and differentiation.
免疫印迹
WB result of E-Cadherin Rabbit mAb (HRP Conjugate)
Primary antibody: E-Cadherin Rabbit mAb (HRP Conjugate) at 1/1000 dilution
Lane 1: MDA-MB-231 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Negative control: MDA-MB-231 whole cell lysate
Predicted MW: 97 kDa
Observed MW: 80~125 kDa
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