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宿主来源
Mouse抗原名称
CD28分子别名
TP44细胞定位
MembraneAccession
P10747克隆号
15E8抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu纯化方式
Protein G浓度
0.2mg/ml标记
Alexa Fluor® 594性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
FCM
稀释度
应用 稀释度 FCM 5μl per million cells in 100μl volume
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.
流式分析
- Flow cytometric analysis of Human CD28 expression on Jurkat. Cells from the Jurkat (Human T cell leukemia T lymphocyte, Right) or THP-1 (Human monocytic leukemia monocyte, Left) was stained with Alexa Fluor® 594 Mouse IgG1, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 594 Mouse Anti-Human CD28 Antibody (15E8) (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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