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宿主来源
Rabbit抗原名称
HSP60分子别名
60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein细胞定位
Mitochondrion MatrixAccession
P10809克隆号
SDT-R012抗体类型
Recombinant mAb反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.应用
ICFCM
ICC ?
稀释度
应用 稀释度 推荐种属 ICC 1:500 Hu, Ms, Rt
HSP60, also known as chaperonins (Cpn), is a family of heat shock proteins originally sorted by their 60kDa molecular mass. They prevent misfolding of proteins during stressful situations such as high heat, by assisting protein folding. HSP60 belongs to a large class of molecules that assist protein folding, called molecular chaperones.
流式分析
Flow cytometric analysis of HeLa cells labelling HSP60 (Alexa Fluor 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of C6 cells labelling HSP60 (Alexa Fluor 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-HSP60 (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in NIH/3T3 cells. Anti-HSP60 (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in C6 cells. Anti-HSP60 (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
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