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宿主来源
Rabbit抗原名称
Antithrombin III分子别名
ATIII, Serpin C1免疫原
Recombinant Protein细胞定位
Extracellular space, SecretedAccession
P01008克隆号
SDT-294-211抗体类型
Recombinant mAb反应种属 ?
Hu交叉反应
Does not recognize thrombin, PIC complex, tPA-PAI-1 complex纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 WB 1:1000 IP 1:50
Antithrombin III (AT III) is primarily a potent anticoagulant with independent anti-inflammatory properties. AT III irreversibly inhibits serine proteases (e.g. activated factor X and thrombin) in a one-to-one ratio, with the generation of protease–AT III complexes. Heparin prevents AT III from interacting with the endothelial cell surface by binding to sites on the AT III molecule, competing for the AT III binding site, and reducing AT III’s ability to interact with its cellular receptor [PMID: 26862016].
免疫印迹
WB result of Antithrombin III Rabbit mAb
Primary antibody: Antithrombin III Rabbit mAb at 1/1000 dilution
Lane 1: Human serum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52kDa
Observed MW: 55kDa
免疫沉淀
Antithrombin III Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Antithrombin III in 0.4 mg human serum lysate.
Western blot was performed on the immunoprecipitate using Antithrombin III Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: human serum lysate 10 µg (Input)
Lane 2: Antithrombin III Rabbit mAb IP in human serum lysate
Lane 3: Rabbit monoclonal IgG IP in human serum lysate
Predicted MW: 52 kDa
Observed MW: 55 kDa
（This blot was developed with high sensitivity substrate）
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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