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Antithrombin III Recombinant Rabbit mAb (SDT-294-211)

ATIII,Serpin C1

货号： S0B0106

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Antithrombin III

  • 分子别名

    ATIII, Serpin C1

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted, Extracellular space

  • Accession

    P01008

  • 克隆号

    SDT-294-211

  • 抗体类型

    Recombinant mAb

  • 应用

    WB, IP

  • 反应种属 ?

    Hu

  • 交叉反应

    Does not recognize thrombin, PIC complex, tPA-PAI-1 complex

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标签

    N/A

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度
WB 1:1000
IP 1:50
背景介绍

  • Antithrombin III (AT III) is primarily a potent anticoagulant with independent anti-inflammatory properties. AT III irreversibly inhibits serine proteases (e.g. activated factor X and thrombin) in a one-to-one ratio, with the generation of protease–AT III complexes. Heparin prevents AT III from interacting with the endothelial cell surface by binding to sites on the AT III molecule, competing for the AT III binding site, and reducing AT III’s ability to interact with its cellular receptor [PMID: 26862016].

  • 免疫印迹

    • WB result of Antithrombin III Rabbit mAb
      Primary antibody: Antithrombin III Rabbit mAb at 1/1000 dilution
      Lane 1: Human serum lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52kDa
      Observed MW: 55kDa

  • 免疫沉淀

    • Antithrombin III Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Antithrombin III in 0.4 mg human serum lysate.
      Western blot was performed on the immunoprecipitate using Antithrombin III Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: human serum lysate 10 µg (Input)
      Lane 2: Antithrombin III Rabbit mAb IP in human serum lysate
      Lane 3: Rabbit monoclonal IgG IP in human serum lysate
      Predicted MW: 52 kDa
      Observed MW: 55 kDa
      （This blot was developed with high sensitivity substrate）

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