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Mouse抗原名称
ALPHA SMOOTH MUSCLE ACTIN分子别名
Alpha-actin-2, ACTA2, SMA细胞定位
Cytoplasm, CytoskeletonAccession
P62736克隆号
SDT-R142抗体类型
Recombinant mAb抗体同种型
IgG2a反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Dg纯化方式
Protein A浓度
0.4 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Ms IHC-P 1:4000 Hu, Ms, Rt ICC 1:100 Hu ICFCM 1:400 Hu IP 1:40 Ms
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
免疫印迹
WB result of alpha smooth muscle Actin mouse mAb
Primary antibody: alpha smooth muscle Actin mouse mAb at 1/10000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse stomach lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42kDa
Observed MW: 42kDaWB result of alpha smooth muscle Actin mouse mAb
Primary antibody: alpha smooth muscle Actin mouse mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42kDa
Observed MW: 42kDa
流式分析
Flow cytometric analysis of HeLa cells labelling alpha smooth muscle Actin antibody at 1/400 (0.1 μg) dilution/ (red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
alpha smooth muscle Actin Mouse mAb at 1/40 dilution (1 µg) immunoprecipitating alpha smooth muscle Actin in 0.4 mg NIH/3T3 whole cell lysate.
Western blot was performed on the immunoprecipitate using alpha smooth muscle Actin Mouse mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: NIH/3T3 whole cell lysate 20 µg (Input)
Lane 2: alpha smooth muscle Actin Mouse mAb IP in NIH/3T3 whole cell lysate
Lane 3: Mouse monoclonal IgG2a IP in NIH/3T3 whole cell lysate
Predicted MW: 42 kDa
Observed MW: 42 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-alpha smooth muscle Actin antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI. Counterstain with tubulin (red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
引用文献(1)
- Procyanidin C1 ameliorates aging-related skin fibrosis through targeting EGFR to inhibit TGFβ/SMAD pathway
JH Wang, M Li, PF Xie, JY Si, ZJ Feng, CF Tang, JM Li
Phytomedicine. 2025 Apr 17 ; 1
影响因子： 6.7
货号：S0B0102产品名称：alpha smooth muscle Actin Recombinant Mouse mAb (SDT-R142)
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