STING Recombinant Rabbit mAb (SDT-R150)

Hsting,ERIS,hMITA,Transmembrane protein 173

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0084

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

STING
分子别名

Hsting, ERIS, hMITA, Transmembrane protein 173
细胞定位

Cytoplasm, Cell membrane, Endoplasmic reticulum membrane, Mitochondrion outer membrane
Accession

Q86WV6
克隆号

SDT-R150
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度

WB 1:1000
IHC-P 1:500
IP 1:50

应用	稀释度
WB	1:1000
IHC-P	1:500
IP	1:50

背景介绍

Mediator of IRF3 activation (MITA, also known as stimulator of interferon genes (STING) and endoplasmic reticulum interferon stimulator (ERIS)) is an ER-associated protein that senses cellular and bacterium-derived cyclic dinucleotide (CDN), leading to induction of type-I interferons (IFNs) and innate immune responses against viruses and bacteria [PMID: 36193584].

免疫印迹
- WB result of STING Rabbit mAb Primary antibody: STING Rabbit mAb at 1/1000 dilution Lane 1: THP-1 whole cell lysate 20 µg Lane 2: HaCaT whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42kDa Observed MW: 30, 37kDa Exposure time: 180s
免疫沉淀
- STING Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating STING in 0.4 mg HL-60 whole cell lysate.
  Western blot was performed on the immunoprecipitate using STING Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/400 dilution.
  Lane 1: HL-60 whole cell lysate 20 µg (Input)
  Lane 2: STING Rabbit mAb IP in HL-60 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HL-60 whole cell lysate
  Predicted MW: 42 kDa
  Observed MW: 37 kDa
  Exposure time: 180 s
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.