β-actin Recombinant Rabbit mAb (FITC conjugate) (SDT-R015)

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货号： S0B0078

Datasheet COA

价格： ￥240
规格：
10μl25μl100 μl1ml
数量：

大包装询价

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产品规格

宿主来源

Rabbit
抗原名称

β-Actin
分子别名

ACTB
免疫原

N/A
细胞定位

Cytoplasm, Cytoskeleton
Accession

P60709
克隆号

SDT-R015
抗体类型

Recombinant mAb
应用

ICFCM, ICC
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

1 mg/ml
标记

FITC
性状

Liquid
缓冲体系

PBS, 0.1% BSA, 0.01% Proclin 300
储存条件

12 months from date of receipt / reconstitution, 4 °C as supplied.

稀释度

应用	稀释度
ICC	1:100

背景介绍

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

流式分析
- Flow cytometric analysis of HeLa cells labelling β-actin (FITC Conjugate) antibody at 1/1000 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
- Flow cytometric analysis of NIH/3T3 cells labelling β-actin (FITC Conjugate) antibody at 1/100 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in HeLa cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in NIH/3T3 cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in C6 cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
- ICC shows positive staining in NIH/3T3 cells. Anti-β-actin (FITC conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).