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产品规格
宿主来源
Rabbit抗原名称
alpha smooth muscle Actin (SMA)分子别名
Alpha-actin-2,ACTA2免疫原
Synthetic PeptideAccession
P62736克隆号
SDT-132-63抗体类型
Recombinant mAb反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Cy5性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
IF ?
稀释度
应用 稀释度 IF 1:100
背景介绍
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas α-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in non-muscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
免疫组化
- IF shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Cy5 Conjugate) antibody was used at 1/100 dilution (magenta). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.







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