β-actin Recombinant Rabbit mAb (SDT-R156)

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货号： S0B0074

Datasheet COA

价格： ￥400
规格：
25ul100 μl1ml
数量：

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

β-Actin
分子别名

ACTB
免疫原

N/A
细胞定位

Cytoskeleton, Cytoplasm
Accession

P60709
克隆号

SDT-R156
抗体类型

Recombinant mAb
应用

ICFCM, IHC-P, ICC, WB
反应种属 ?

Hu, Ms
纯化方式

Protein A
浓度

0.5 mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度
WB	1:1000-1:5000
IHC-P	1:2000
ICC	1:500
ICFCM	1:500

背景介绍

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

免疫印迹
- WB result of β-actin Rabbit mAb Primary antibody: β-actin Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: A549 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42kDa Observed MW: 42kDa
- WB result of β-actin Rabbit mAb Primary antibody: β-actin Rabbit mAb at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Lane 2: mouse kidney lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42kDa Observed MW: 42kDa
流式分析
- Flow cytometric analysis of HeLa cells labelling β-actin antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of NIH/3T3 cells labelling β-actin antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-β-actin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
- ICC shows positive staining in NIH/3T3 cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).

引用文献(1)

Cancer-associated adipocytes mediate CD8+ T cell dysfunction via FGF21-driven lipolysis
S Dalangood, C Hu, C Yuan, X Li, W Qiao, H Li

BioRxiv. 2024 Oct 21 .

货号：S0B0074产品名称：β-actin Recombinant Rabbit mAb (SDT-R156)