Laminin gamma 1/LAMC1 Recombinant Rabbit mAb (SDT-148-80)
Laminin B2 chain,Laminin-1 subunit gamma,Laminin-10 subunit gamma,Laminin-11 subunit gamma,Laminin-2 subunit gamma,Laminin-3 subunit gamma,Laminin-4 subunit gamma,Laminin-6 subunit gamma,Laminin-7 subunit gamma,Laminin-8 subunit gamma,Laminin-9 subunit gamma,S-laminin subunit gamma,S-LAM gamma
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宿主来源
Rabbit抗原名称
lL-8分子别名
Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma, S-LAM gamma免疫原
Recombinant Protein细胞定位
Basement membrane, Secreted, Extracellular matrixAccession
P11047克隆号
SDT-148-80反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied应用
WB
稀释度
应用 稀释度 WB 1:1000-1:20000
Laminins are large molecular weight glycoproteins constituted by the assembly of three disulfide-linked polypeptides, the α, β and γ chains. The human genome encodes 11 genetically distinct laminin chains. Structurally, laminin chains differ by the number, size and organization of a few constitutive domains, endowing the various members of the laminin family with common and unique important functions. In particular, laminins are indispensable building blocks for cellular networks physically bridging the intracellular and extracellular compartments and relaying signals critical for cellular behavior, and for extracellular polymers determining the architecture and the physiology of basement membranes [PMID: 23263632].
免疫印迹
- WB result of LAMC1 Rabbit mAb Primary antibody: LAMC1 Rabbit mAb at 1/1000 dilution Lane 1: A431 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 178 kDa Observed MW: 250 kDa
- WB result of LAMC1 Rabbit mAb Primary antibody: LAMC1 Rabbit mAb at 1/5000 dilution Lane 1: mouse testis lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 178 kDa Observed MW: 250 kDa
- WB result of LAMC1 Rabbit mAb Primary antibody: LAMC1 Rabbit mAb at 1/20000 dilution Lane 1: mouse placenta lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 178 kDa Observed MW: 250 kDa
- WB result of LAMC1 Rabbit mAb Primary antibody: LAMC1 Rabbit mAb at 1/5000 dilution Lane 1: rat stomach lysate 20 µg Lane 2: C6 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 178 kDa Observed MW: 250 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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