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产品规格
宿主来源
Rabbit抗原名称
CD226分子别名
DNAM-1, TLisA1, PTA-1免疫原
Recombinant Protein细胞定位
Cell membraneAccession
Q15762克隆号
SDT-284-30应用
IHC-P, WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:1000 |
背景介绍
CD226, namely T lineage specific activation antigen 1 (TLisA1), platelet and T cell antigen 1 (PTA-1), or DNAM-1, is a member of the immunoglobulin superfamily. It is a member of the immunoglobulin superfamily, and a functional protein initially expressed on natural killer and T cells [PMID: 32850777].
免疫印迹
WB result of CD226 Rabbit mAb Primary antibody: CD226 Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 39 kDa Observed MW: 55~65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD226 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bone marrow. Anti-CD226 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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