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宿主来源
Rabbit抗原名称
SPARC分子别名
BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine细胞定位
Basement membrane, Secreted, Extracellular matrixAccession
P09486克隆号
SDT-R136反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 WB 1:1000 IHC-P 1:2000 IP 1:50
SPARC, also referred to as osteonectin or basement membrane protein 40 (BM-40), is a ~32 kDa calcium-binding matricellular protein [PMID: 22249026, PMID: 25064829]. At one time SPARC was thought to be expressed specifically in mineralized tissues (thus the name osteonectin) but was subsequently demonstrated to have a much wider expression pattern in both mineralized and non-mineralized tissues [PMID: 7034958]. Typically, expression of SPARC is closely aligned with that of fibrillar collagens such as collagen type I. The protein component of the pre-mineralized bone matrix (osteoid) is composed mainly of collagen type I (94%) and a number of noncollagenous proteins.
免疫印迹
- WB result of SPARC Rabbit mAb Primary antibody: SPARC Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: SH-SY5Y whole cell lysate 20 µg Lane 3:A-375 whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 35 kDa Observed MW: 37 kDa Exposure time: 180s
免疫沉淀
SPARC Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SPARC in 0.4 mg A375 whole cell lysate.
Western blot was performed on the immunoprecipitate using SPARC Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A375 whole cell lysate 20 µg (Input)
Lane 2: SPARC Rabbit mAb IP in A375 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A375 whole cell lysate
Predicted MW: 35 kDa
Observed MW: 37 kDa
Exposure time: 120 s
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human kidney. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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