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产品规格
宿主来源
Mouse抗原名称
Tau分子别名
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau免疫原
N/A细胞定位
Cell membrane, Cytoplasm, SecretedAccession
P10636克隆号
SDT-R145抗体同种型
IgG1应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt纯化方式
Protein G浓度
2 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:2000 |
背景介绍
Tau is a microtubule-associated protein for which the physiological functions remain a topic of vigorous investigation. Additionally, tau is a central player in the pathogenesis of several diseases such as Alzheimer’s disease and several frontotemporal dementias. A critical variable to understanding tau in physiological and disease contexts is its normal localization within cells of the adult CNS.
免疫印迹
WB result of Tau mouse mAb Primary antibody: Tau mouse mAb at 1/1000 dilution Lane 1: mouse liver lysate 20 µg Lane 2: mouse brain lysate 20 µg Negative control: mouse liver lysate Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50~65 kDa Observed MW: 50~65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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