CD38 Recombinant Rabbit mAb (SDT-290-45)

2'-phospho-ADP-ribosyl cyclase,2'-phospho-cyclic-ADP-ribose transferase,ADPRC 1,cADPR hydrolase 1,I-19,NIM-R5 antigen

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0058

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD38
分子别名

2'-phospho-ADP-ribosyl cyclase, 2'-phospho-cyclic-ADP-ribose transferase, ADPRC 1, cADPR hydrolase 1, I-19, NIM-R5 antigen
免疫原

Synthetic Peptide
细胞定位

Membrane
Accession

P56528
克隆号

SDT-290-45
抗体类型

Rabbit mAb
反应种属 ?

Ms
纯化方式

Protein A
浓度

0.05mg/ml
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP

IF ?
稀释度

应用稀释度

IHC-P 1:1600
WB 1:1000
IF 1:2000
IP 1:25

应用	稀释度
IHC-P	1:1600
WB	1:1000
IF	1:2000
IP	1:25

背景介绍

CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 functions in cell adhesion, signal transduction and calcium signaling. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications including social amnesia possibly related to autism. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism, as well as some genetically determined conditions.

免疫印迹
- WB result of CD38 Rabbit mAb Primary antibody: CD38 Rabbit mAb at 1/1000 dilution Lane 1: mouse spleen lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 34 kDa Observed MW: 39 kDa Exposure time: 180s
- WB result of CD38 Rabbit mAb Primary antibody: CD38 Rabbit mAb at 1/1000 dilution Lane 1: mouse colon lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 34 kDa Observed MW: 39 kDa Exposure time: 60s
免疫沉淀
- CD38 Rabbit mAb at 1/25 dilution (0.2 µg) immunoprecipitating CD38 in 0.4 mg mouse spleen lysate.
  Western blot was performed on the immunoprecipitate using CD38 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: mouse spleen lysate 10 µg (Input)
  Lane 2: CD38 Rabbit mAb IP in mouse spleen lysate
  Lane 3: Rabbit monoclonal IgG IP in mouse spleen lysate
  Predicted MW: 34 kDa
  Observed MW: 39 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle . Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD38 antibody was used at 1/1600 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD38 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.