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宿主来源
Rabbit抗原名称
Hemoglobin分子别名
Hb免疫原
Native protein细胞定位
Cytosol, Blood microparticleAccession
P69905, P68871克隆号
SDT-193-71抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.25 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 WB 1:500 IHC-P 1:5000 IP 1:25
The primary function of Hemoglobin (Hb) is to transport oxygen (O2) from the lung to tissues, binding and releasing O2 in a cooperative manner [PMID: 32189307]. Hemoglobin is a tetramer, consisting of two α-subunits (α1 and α2) and two βsubunits (β1 and β2) that are structurally similar and about the same size. The two αβ dimers (named α1β1 and α2β2) are arranged around a 2-fold axis of symmetry resulting in a large central water cavity in the T or unliganded or deoxygenated structure and a narrower cavity in the R or liganded or oxygenated structure.
免疫印迹
- WB result of Hemoglobin Rabbit mAb Primary antibody: Hemoglobin Rabbit mAb at 1/500 dilution Lane 1: 293T whole cell lysate 20 µg Lane 2: TF-1 whole cell lysate 20 µg Negative control: 293T whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 13 kDa Observed MW: 13 kDa
免疫沉淀
Hemoglobin Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating Hemoglobin in 0.4mg TF-1 whole cell lysate.
Western blot was performed on the immunoprecipitate using Hemoglobin Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : TF-1 whole cell lysate 20µg(input)
Lane 2 : Hemoglobin Rabbit mAb IP in TF-1 whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in TF-1 whole cell lysate
Predicted MW: 13 kDa
Observed MW: 13 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human placenta. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human prostate. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human stomach. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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