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宿主来源
Rabbit抗原名称
Thrombomodulin分子别名
TM, Fetomodulin, CD141免疫原
Recombinant Protein细胞定位
MembraneAccession
P07204克隆号
SDT-153-89抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.25 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 WB 1:5000 IHC-P 1:1000 IP 1:25 ICFCM 1:250 ICC 1:250
Thrombomodulin (TM) is an integral component of a multimolecular system, localized primarily to the vascular endothelium, that integrates crucial biological processes and biochemical pathways, including those related to coagulation, innate immunity, inflammation, and cell proliferation. These are designed to protect the host from injury and promote healing [PMID: 29866818].
免疫印迹
WB result of Thrombomodulin Rabbit mAb
Primary antibody: Thrombomodulin Rabbit mAb at 1/5000 dilution
Lane 1: A431 whole cell lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 90 kDa
Exposure time: 180s
流式分析
Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling Thrombomodulin antibody at 1/250 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
Thrombomodulin Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating Thrombomodulin in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using Thrombomodulin Rabbit mAb at 1/5000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A431 whole cell ly-sate 10 µg (Input)
Lane 2: Thrombomodulin Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 60 kDa
Observed MW: 90 kDa
Exposure time: 20 s
免疫组化
IHC shows positive staining in paraffin-embedded human cervcial squamous cell carcinoma. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A431 cells. Anti-Thrombomodulin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanoland permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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