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Tau (phospho T231) Recombinant Rabbit mAb (SDT-177-1)

p-Tau231,phospho T231,Phospho-Tau (Thr231)

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0054
规格
数量
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产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Tau (phospho T231)

  • 分子别名

    p-Tau231, phospho T231, Phospho-Tau (Thr231)

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane, Cytoplasm, Secreted

  • Accession

    P10636-8

  • 克隆号

    SDT-177-1

  • 抗体类型

    Rabbit mAb

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Ms

  • 预测反应种属
    (反应种属缩写表)

    Cz, Gor, Pr, Or, Hu, Mq

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB ,
    IP

  • 稀释度

    应用 稀释度
    WB 1:1000
    IP 1:50
背景介绍

  • The functions of the neuronal microtubule-associated protein Tau in the central nervous system are regulated by manifold posttranslational modifications at more than 50 sites. Tau in healthy neurons carries multiple phosphate groups, mostly in its microtubule assembly domain. Elevated phosphorylation and aggregation of Tau are widely considered pathological hallmarks in Alzheimer's disease (AD) and other tauopathies, triggering the quest for Tau posttranslational modifications in the disease context [PMID: 33892381]. Tau phosphorylation at Thr231 can be detected in cerebrospinal fluid and shows potential as a biomarker of neurofibrillary pathology in Alzheimer’s disease [PMID: 17012293].

  • 免疫印迹

    • WB result of Tau(phospho T231) Rabbit mAb
      Primary antibody: Tau(phospho T231) Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 5 µg
      Lane 2: mouse brain lysate(phosphatase treated) 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50~70 kDa
      Observed MW: 50~70 kDa
      Exposure time: 60s

  • 免疫沉淀

    • Tau (phospho T231) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Tau (phospho T231) in 0.4 mg mouse brain lysate.
      Western blot was performed on the immunoprecipitate using Tau (phospho T231) Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1: mouse brain lysate 20 µg (Input)
      Lane 2: Tau (phospho T231) Rabbit mAb IP in mouse brain lysate
      Lane 3: Rabbit monoclonal IgG IP in mouse brain lysate
      Predicted MW: 50~70 kDa
      Observed MW: 50~70 kDa
      Exposure time: 150 s（This blot was developed with high sensitivity substrate）

  • 斑点杂交

    • Dot blot result of Tau (phospho T231) Rabbit mAb
      Lane 1: Tau (phospho T231) peptide(aa224~240)
      Lane 2: Tau non-phospho peptide(aa224~240)
      Primary antibody: Tau(phospho T231) Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Exposure time: 8s

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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