热搜词：

CN

  • CN
  • EN

注册

产品中心 抗体 其他一抗

所有图片（1/6）

PG II/PGC Recombinant Rabbit mAb (SDT-194-100)

Gastricsin,Pepsinogen C

货号： S0B0048

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
申请试用 斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    PG II/PGC

  • 分子别名

    Gastricsin, Pepsinogen C

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Secreted

  • Accession

    P20142

  • 克隆号

    SDT-194-100

  • 抗体类型

    Rabbit mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.25 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied
稀释度
应用 稀释度
IHC-P 1:1000
WB 1:2500
背景介绍

  • Pepsinogen (PG) is the precursor of pepsin and is composed of five hypotypes: pepsinogen A (PGA), pepsinogen B (PGB), pepsinogen C (PGC), pepsinogen F (PGF) and chymosinogen [PMID: 28546787]. Pepsinogen C, also known as progastricsin or PG II, is an aspartic protease expressed primarily in gastric chief cells [PMID: 14578117].

  • 免疫印迹

    • WB result of PG II/PGC Rabbit mAb
      Primary antibody: PG II/PGC Rabbit mAb at 1/2500 dilution
      Lane 1: mouse liver lysate 20 µg
      Lane 2: mouse stomach lysate 20 µg
      Negative control: mouse liver lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 38 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-PG II/PGC antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    评论(0)