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Rabbit抗原名称
Claudin 18.2分子别名
CLDN18.2免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P56856-2克隆号
SDT-102-24抗体类型
Rabbit mAb应用
IF反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1.0 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 |
|---|---|
| IF | 1:100 |
Claudin-18 is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. Isoform 2 (Claudin 18.2) is abundant in gastric tumors. Claudin 18.2 is involved in tumor development and progression and located in the outer cell membrane. It has exposed extracellular loops and is available for monoclonal antibody binding.
免疫荧光
IF shows positive staining in paraffin-embedded human stomach. Anti-Claudin 18.2 (FITC Conjugate) antibody was used at 1/100 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse stomach. Anti-Claudin 18.2 (FITC Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat stomach. Anti-Claudin 18.2 (FITC Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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