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α-tubulin Recombinant Rabbit mAb (SDT-R091)

Tubulin alpha-4A chain,Alpha-tubulin 1

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0043
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    α-tubulin

  • 分子别名

    Tubulin alpha-4A chain, Alpha-tubulin 1

  • 细胞定位

    Cytoskeleton, Cytoplasm

  • Accession

    P68366

  • 克隆号

    SDT-R091

  • 抗体类型

    Rabbit mAb

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.1 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.

  • 应用

    IHC-P ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:4000-1:20000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
背景介绍

  • Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.

  • 免疫印迹

    • WB result of α-tubulin Rabbit mAb
      Primary antibody: α-tubulin Rabbit mAb at 1/4000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HepG2 whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 52 kDa

    • WB result of α-tubulin Rabbit mAb
      Primary antibody: α-tubulin Rabbit mAb at 1/4000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse brain lysate 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 52 kDa

    • WB result of α-tubulin Rabbit mAb
      Primary antibody: α-tubulin Rabbit mAb at 1/4000 dilution
      Lane 1: rat brain lysate 5 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 52 kDa

    • WB result of α-tubulin Rabbit mAb
      Primary antibody: α-tubulin Rabbit mAb at 1/20000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 52 kDa
      Observed MW: 52 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-α-tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-α-tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-α-tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-α-tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-α-tubulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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