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宿主来源
Rabbit抗原名称
β-tubulin (FITC Conjugate)分子别名
Tubulin beta chain细胞定位
Cytoplasm, CytoskeletonAccession
P07437克隆号
SDT-R014抗体类型
Rabbit mAb反应种属 ?
Hu, Ms纯化方式
Protein A浓度
1.0 mg/ml性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ICC ?
稀释度
应用 稀释度 ICFCM 1:50 ICC 1:200
β-tubulin is a 55 kDa protein that forms a heterodimer with alpha tubulin to make tubulin, the primary component of microtubules. Microtubules are rigid, hollow fibers that make up the cell's structural framework (the cytoskeleton).
流式分析
Flow cytometric analysis of HeLa cells labelling β-tubulin (FITC Conjugate) antibody at 1/50 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Flow cytometric analysis of NIH/3T3 cells labelling β-tubulin (FITC Conjugate) antibody at 1/50 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-β-tubulin (FITC Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
ICC shows positive staining in NIH/3T3 cells. Anti-β-tubulin (FITC Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
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