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CDK6 Recombinant Rabbit mAb (SDT-R047)

Cyclin-dependent kinase 6,CDKN6

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0037
规格
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产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CDK6

  • 分子别名

    Cyclin-dependent kinase 6, CDKN6

  • 免疫原

    N/A

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    Q00534

  • 克隆号

    SDT-R047

  • 抗体类型

    Rabbit mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICC ? ,
    WB ,
    IP

  • 稀释度

    应用 稀释度
    WB 1:1000
    IP 1:25
    ICC 1:500
背景介绍

  • CDK6 is a key regulator during the G1/S cell cycle transition. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types. Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans.

  • 免疫印迹

    • WB result of CDK6 Rabbit mAb
      Primary antibody: CDK6 Rabbit mAb at 1/1000 dilution
      Lane 1: MCF7 whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: MOLT-4 whole cell lysate 20 µg
      Negative control: MCF7 whole cell lysate

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 36 kDa
      Observed MW: 36 kDa
      Exposure time: 10s

  • 免疫沉淀

    • CDK6 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CDK6 in 0.4mg MOLT-4 whole cell lysate.
      Western blot was performed on the immunoprecipitate using CDK6 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/400 dilution.
      Lane 1 : MOLT-4 whole cell lysate 10µg(input)
      Lane 2 : CDK6 Rabbit mAb IP in MOLT-4 whole cell lysate
      Lane 3 : Rabbit monoclonal IgG IP in MOLT-4 whole cell lysate
      Predicted MW: 36 kDa
      Observed MW: 36 kDa
      Exposure time: 20s

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- CDK6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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