分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
PLK1分子别名
STPK13细胞定位
NucleusAccession
P53350克隆号
SDT-R062抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
1.0 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 IHC-P 1:200 WB 1:20000 IP 1:100
PLK1 is a serine/threonine kinase that plays a pivotal role in cell division. Its dysregulation is tightly linked to malignant transformation. In human cancers, there is growing evidence coupling PLK1 activity to tumor development, progression, and therapy resistance. PLK1 is overexpressed in various cancers, and its expression levels correlate with poor prognosis. During cell division, the most prominent network or chestrating mitotic onset consists of the PLK1-Aurora A-Cyclin B1/CDK1 feedback loop. In this respect, PLK1 activity is reported to be a critical prerequisite for mitotic entry.
免疫印迹
WB result of PLK1 Rabbit mAb
Primary antibody: PLK1 Rabbit mAb at 1/20000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K-562 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 62 kDa
免疫沉淀
PLK1 Rabbit mAb at 1/100 dilution (1µg) immunoprecipitating PLK1 in 0.4mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using PLK1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : K562 whole cell lysate 10µg(input)
Lane 2 : PLK1 Rabbit mAb IP in K562 whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 68 kDa
Observed MW: 62 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PLK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PLK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PLK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-PLK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)